TsCDase CAS# EC:3.2.1.54

Identification

CAS Number

None

Name

TsCDase

Molecular weight

72kD

EC Number

3.2.1.54

Properties

Appearance

Colorless to light brown liquid

Safety Data

RIDADR 

NONH for all modes of transport

Specifications and Other Information of Our TsCDase CAS# EC:3.2.1.54

Product Description

The TsCDase gene is derived from Thermococcus sp. Strain B1001 (EC: 3.2.1.54) and is recombinantly expressed in Escherichia coli.
It catalyzes the hydrolysis of cyclodextrins into maltose; specifically, α-cyclodextrin is hydrolyzed into maltooctaose, β-cyclodextrin into maltoheptaose, and γ-cyclodextrin into maltohexaose.

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol of (Glcα1,4)₆Glc from β-cyclodextrin per minute at 80°C and pH 5.0.

Purity

＞80%

Activity

≥1000U/ml

Suggested Usage

  1. Add the substrate to the reaction system; a buffer salt may be added as appropriate to adjust the pH to 5.0. Add a suitable amount of TsCDase (based on reaction requirements).
  2. After mixing thoroughly, incubate the sample at 70°C for 2–4 hours.
  3. To terminate the reaction, add 50% anhydrous ethanol, mix by stirring, and then centrifuge and filter the solution.
    [Note] When scaling up the reaction system, the incubation time or enzyme dosage should be adjusted according to actual conditions; low-speed shaking may be used for mixing.

Storage Buffer

20mM Tris-HCl, pH 7.5, 200mM NaCl,10% glycerol.

Shelf Life

2 years

Storage

-20°C with sealed well is 18 months.

This product is developed by our R&D company Watson Bio Ltd (https://www.watson-bio.com/).

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).