Structure of Recombinant Carboxypeptidase B EC 3.4.17.2 CAS 9025-24-5

Recombinant Carboxypeptidase B EC 3.4.17.2 CAS 9025-24-5

Identification

CAS Number

9025-24-5

EC Number

3.4.17.2

Name

Recombinant Carboxypeptidase B

Synonyms

N-(3-Carboxypropanoyl)alanylalanyl-N-[1-(benzylsulfanyl)-1-oxo-3-phenyl-2-propanyl]prolinamid [German] [ACD/IUPAC Name]
N-(3-Carboxypropanoyl)alanylalanyl-N-[1-(benzylsulfanyl)-1-oxo-3-phenyl-2-propanyl]prolinamide [ACD/IUPAC Name]
N-(3-Carboxypropanoyl)alanylalanyl-N-[1-(benzylsulfanyl)-1-oxo-3-phényl-2-propanyl]prolinamide [French] [ACD/IUPAC Name]
Prolinamide, N-(3-carboxy-1-oxopropyl)alanylalanyl-N-[2-oxo-1-(phenylmethyl)-2-[(phenylmethyl)thio]ethyl]- [ACD/Index Name]
3-[(1-{[1-(2-{[1-(BENZYLSULFANYL)-1-OXO-3-PHENYLPROPAN-2-YL]CARBAMOYL}PYRROLIDIN-1-YL)-1-OXOPROPAN-2-YL]CARBAMOYL}ETHYL)CARBAMOYL]PROPANOIC ACID
4-[[1-[[1-[2-[(1-benzylsulfanyl-1-oxo-3-phenylpropan-2-yl)carbamoyl]pyrrolidin-1-yl]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobutanoic acid
9025-24-5 [RN]
Carboxypeptidase B from human pancreas
Carboxypeptidase- B porcine pancreas
Protaminase

SMILES

CC(C(=O)NC(C)C(=O)N1CCCC1C(=O)NC(Cc2ccccc2)C(=O)SCc3ccccc3)NC(=O)CCC(=O)O

InChi

InChI=1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)

InChIKey

TWURVFFNODFJBJ-UHFFFAOYSA-N

Molecular Formula

C31H38N4O7S

Molecular Weight

610.721

EINECS

232-788-9

MDL Number

MFCD00081476

Properties

Appearance

White or off white, or yellowish powder

Safety Data

Symbol

exclamation-mark-jpgGHS07,GHS08

Signal Word

Danger

Hazard statements

H315 – H319 – H334 – H335

Precautionary Statements

P302 + P352 – P305 + P351 + P338

Target organs

Respiratory system

Personal Protective Equipment

Eyeshields, Gloves

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our Recombinant Carboxypeptidase B EC 3.4.17.2 CAS 9025-24-5

ItemsRecombinant Carboxypeptidase BSequencing Grade Carboxypeptidase B
Specific activity≥170 units/mg pro.≥200 units/mg pro.
SDS-PAGESingle major bandSingle major band
Molecular Weight(SDS-PAGE) 33.8±3.4 kDa33.8±3.4 kDa
Purity(HPLC)/≥ 95%
Unit definitionOne unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.
Recommended usagePrepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0./

Description

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.

Source

 It is a genetically engineered protein expressed in E.coli

Protein Sequence

The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B

Stability of storage

Sequencing grade carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃. Avoid repeated freezing and thawing.

Stability of transport

The product is stable by blue ice insulation transport.

Advantage

  1. Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
  2. Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
  3. High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
  4. Lyophilized powder: The product is lyophilized powder and is easy to store and transport.

Known Application

The production of recombinant insulin and its analogues; the quality test of recombinant antibodies; the determination of C-terminal amino acids of proteins; the production of other recombinant polypeptides; and the enzymatic synthesis of certain specific compounds. Determination of protein C-terminal basic amino acids such as arginine, lysine and histidine; detection of antibody basic peak.

Watson Bio Ltd

This product is developed by our sub R&D company Watson Bio Ltd, and please click the link below for details. https://www.watson-bio.com/recombinant-soybean-trypsin-inhibitor-cas-9035-81-8/

Watson International Ltd

This product is sold exclusively through our sales company Watson International Ltd, and please click the link below for details. http://www.watson-int.com/recombinant-soybean-trypsin-inhibitor-cas-9035-81-8/