Structure of Monoamine Oxidase CAS 9001-66-5

KAOD(Monoamine Oxidase) CAS 9001-66-5

Identification

CAS Number

9001-66-5

Name

Monoamine Oxidase

EC Number

1.5.3 (Recombinant from microorganism)

Molecular Formula

C7H15N2O6P

Molecular Weight

55 kDa (SDS-PAGE)

EINECS

232-623-0

Safety Data

WGK Germany

3

Specifications and Other Information of Our Monoamine Oxidase CAS 9001-66-5

Specification Standards
AppearanceYellow amorphous powder, lyophilized
Protein purity≥90% (from SDS-PAGE)
Activity≥4 U/mg solid
Catalase≤0.001%
ATPase≤0.005%
Glucose oxidase≤0.03%
Cholesterol oxidase≤0.003%

General Assay Characteristics

Method
Enzymatic/RATE A/E
ReagentsReady to use
CalibratorsLevel 1-0.0 U/L; Level 1-50 U/L
Calibration Stability28 days
Sample TypeEDTA,SST,Serum,Heparinised plasma
Assay Range0-200U/L undiluted
Standardisation

Assay Principle

The assay is based on the increase in absorbance at 555 nm as the formation of quinoneimine dye proceeds in the forward reactions.

Unit Definition

One unit (U) is defined as the amount of enzyme which produces 1 μmol of H2O2 per min under the conditions described below.

Proposed MAO Recipe

Reagent I

CompositionConcentrationCatalog Number
Tris-HCl buffer50mM
NADH0.45mMCS001
α-Ketoglutarate15mM
Detergent, Preservative

Reagent II

CompositionConcentrationCatalog Number
Tris-HCl buffer50mM
Benzylamine10mM
Detergent,Preservative

Major reagent ingredients made by Assay Specilist Lab are list on right

Standard&Control

Home Made: MAO in buffer

Reaction curve chart

Known Application

The enzyme is useful for the determination of fructosyl-L-amino acid.

This product is developed by our sub R&D company Ulcho Biochemical Ltd, and please click the link below for details. http://www.ulcho.com/monoamine-oxidase-cas-9001-66-5/ This product is sold exclusively through our sales company Watson International Ltd, and please click the link below for details. http://www.watson-int.com/monoamine-oxidase-cas-9001-66-5/