LLCD CAS 9001-60-9
- Identification
- Properties
- Safety Data
- Specifications & Other Information
- Links
Identification
CAS Number
9001-60-9
EC
1.1.1.27
Name
LLCD, L-Lactic dehydrogenase
Source
Microorganism
Molecular weight
38 kDa (SDS-PAGE)
EINECS
232-617-8
Properties
Appearance
White amorphous powder
Isoelectric point
6.2
Michaelis constant
1.3×10 M(Pyruvate)
-6 4.0×10 M(NADH)
Safety Data
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our LLCD CAS 9001-60-9
Specific activity
≥ 300U/mg protein
Unit definition
One unit will convert one micromole of pyruvate to L-lactate per min at pH 7.4 at 25℃.
Optimum pH
6.5
Optimum temperature
45℃
pH Stability
4.5~10.0 (37℃,1hr)
Thermal stability
< 50℃ (pH 7.4, 15min)
Inhibitors
Co ,Cu ,Fe ,Ni ,Zn ,NEM,SDS,Proclin
Effect of Various Chemicals on L-LDH
The enzyme dissolved in 50mM Tris-HCl buffer, pH 7.5 (20U/ml) was incubated with each chemical at 37℃ for 2hr
Chemical | Concn.(mM) | Residual activity |
None | 2.0 | 100% |
CaCl2 | 2.0 | 101% |
CoCl2 | 2.0 | 50% |
CuSO4 | 2.0 | 0% |
FeCl3 | 2.0 | 27% |
MgSO4 | 2.0 | 94% |
MnSO4 | 2.0 | 96% |
NiCl2 | 2.0 | 78% |
ZnSO4 | 2.0 | 0% |
K4Fe(CN)6 | 2.0 | 98% |
BME | 2.0 | 98% |
NME | 2.0 | 79% |
EDTA | 5.0 | 102% |
NaN3 | 20.0 | 101% |
Proclin | 0.045% | 58% |
Na-cholate | 0.10% | 109% |
SDS | 0.05% | 0% |
Triton X-100 | 0.10% | 113% |
Tween 20 | 0.10% | 115% |

Shelf Life
2 years
Storage
Store at -20℃ for long time.
Known Application
For biochemical research, determination of pyruvate, diagnosis of leukemia and myocardial infarction.
Links
This product is developed by our R&D company Ulcho Biochemical Ltd (http://www.ulcho.com/).
This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).