Ascorbate oxidase CAS UENA-0244

Identification

CAS Number

UENA-0244

Name

Ascorbate oxidase

Synonyms

ASCORBASE;
ASCORBATE OXIDASE;
ASCORBATE OXIDASE CUCURBITA SPECIES;
EC 1.10.3.3;
L-ASCORBATE: OXYGEN OXIDOREDUCTASE;
Oxidase, ascorbate;
ASCORBATE OXIDASE FROM CUCURBITASPECIES

Molecular weight

80 kDa (Gel filtration)

Properties

Appearance

Light blue amorphous powder, lyophilized

Safety Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our Ascorbate oxidase CAS UENA-0244

Activity

≥100 U/mg solid

Km

Ascorbic acid (pH 7.0) 1.0×10-4 M
Ascorbic acid (pH 4.0) 3.8×10-4 M

Optimum pH

4.0–4.5 (Acetate buffer)

Optimum temperature

55℃

pH stability

6.0–9.0 (30℃, 24 hr)

Thermal stability

Stable at 50 ℃ and below (pH 7.0,10 min)

SOL. (50mg/ml water)

Corresponds

PH (50mg/ml water)

7.5

ACT. (25°C; with L-ascorbate)

178 u/mg L

SPEC. ACT. (25°C; with L-ascorbate)

2119 u/mg P

ACT. (37°C; with L-ascorbate)

278 u/rng L

SPEC. ACT. (37°C; with L-ascorbate)

3471 u/mg P

PROTEIN (BCA reag.)

0.084 mg/mg L

Contaminating Activities

Corresponds

GOT

<0.00005%

GPT

<0.00006%

Catalase

<0.008%

Contaminating Oxidases

<0.00003%

Storage stability

-20 ℃ for 12 months for more than 90% activity.

Storage

Store at -20°C for long time, sealed and away from light.

Known Application

This enzyme is mainly used to effectively prevent the interference of ascorbic acid on diagnostic tests such as blood, uric acid, TG, TC, and creatinine.

This product is developed by our R&D company Ulcho Biochemical Ltd (http://www.ulcho.com/).

This product is developed by our R&D company Watsonnoke Scientific Ltd (http://www.watsonnoke.com/).